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Postdoc

Post.Doc "Advanced renal cell models and kidney organoids"

The group Ion Transport of the department of Physiology is seeking a Postdoc.

Position
Postdoc

Job profile

Within the Ion Transport group of the department of Physiology, we are seeking a postdoc for a combined genetic, molecular and physiological project entitled which is funded by an VICI grant from ZonMW. We offer a challenging Postdoc project in a well-organized, international, and high-profile department within the Radboud Institute for Molecular Life Sciences.

Over the last years many renal cell models have been developed and characterized in order to study functional ion transport processes. Advanced techniques were implemented to isolate various fluorescent nephron segment using the Complex Object Parametric Analyzer and Sorter (COPAS). This system features a specially engineered fluidic path, flow cell and optics optimized for a specific subset of that size range to provide maximum speed, accuracy, resolution and throughput, depending on the isolation of the renal segments. Isolated tubules can be subsequently seeded on permeable filter supports to study ion transport processes under physiological and pathophysiological conditions. More recently, there are several differentiation methods from human iPSCs/ESCs into embryonic renal progenitor cells, such as those included in metanephric mesenchyme and ureteric bud, that mimic embryonic development have been reported. These studies show the developmental potential of progenitor cells by forming renal tubule-like or glomerulus-like structures in vitro. However, it has not been verified whether the physiological functions of the induced progenitors are equivalent to their in-vivo counterparts. The establishment of definitive marker genes for kidney lineages and functional assay systems is essential for the verification. Such achievement is needed before kidney regeneration can provide cell replacement therapy, reliable disease models and elucidation of the mechanisms of kidney development. Using the above-indicated techniques functionomics can be studied in various nephron segments to characterize new renal cell models including kidney organoids and to understand the molecular regulation of (un)known transporters.

Tasks and responsibilities

  • The postdoc will design and perform scientific research in the area of renal cell systems, kidney organoids, human iPSCs and physiological characterization
  • Generates and analyses the data and writes scientific publications and presents findings at scientific meetings
  • Works in an international team with other scientists to discuss, plan and perform research in a stimulating environment

Requirements

  • Ph.D. degree in (medical) biology or related discipline
  • Experience with cell culture, molecular & cell biology
  • Experience with animal research would be a prĂ©
  • Independent and well-structured working style
  • Strong motivation to succeed in scientific research
  • Well-developed social skills directed to work in a team
  • Interest in transport physiology and clinically-oriented research

Organization

The department of Physiology has a long-standing tradition in transport physiology in epithelia and has build up a substantial know-how and infrastructure in the field of molecular and cellular physiology of water and electrolyte transport. The department is, together with basic science and research-oriented clinical departments of the Radboud University Medical Center, housed in the Radboud Institute for Molecular Life Sciences, which integrates diverse scientific expertises in molecular and medical science for the advancement of innovation in translational research.

Closing date
On going

Further information and contact

Salary is according to CAO-RUMC. Additional information about the vacancy can be obtained from Prof. Joost Hoenderop  or detailed information about the department can also be found at www.physiomics.eu

Applicants should send a letter of intent outlining special interest in the position, overall related qualifications, experience and career goals, a curriculum vitae and names and addresses of professional references to us via this apply button.

 

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